Anodic stripping voltametric cell for determination of copper and lead in atmospheric dust

Studies of air pollution by metals contained in dust particules usually require mineralization of a dust-collecting filter. This treatment introduces impurities into the sample. To avoid such contamination, an anodic stripping cell was constructed requiring no preliminary treatment of the sample filter. The cell uses a three-electrode amperometric system in differential pulse anodic stripping mode (DPAS). Solubilization of the metals from dust by a complexing agent, instead of the strong acid normally encountered in other methods, allows selective determination of metals according to their chemical form. With this cell, minimal handling of the collection filter and a large area mercury-coated vitreous carbon electrode located close to the sample filter (2 mm) lead to limits of detection as low as 0.15 μg of lead and 10 ng of copper. Precision is 2% for lead determination and 4% for copper determination (RSD).     

DIVA: Recognition of typical situations for turbine generator diagnosis

DIVA is an expert system for turbine generator diagnosis. Its aim is to help a plant operator interpret vibration evolution to diagnose developing faults. Interpretation of vibration evolution is primarily a recognition process which is achieved by a particular model of heuristic classification. DIVA tries to recognize the observed situation among a hierarchy of typical situations.The purpose of this paper is to present the model we used for heuristic classification, based on prototypes. Prototypes represent typical problem situations and may also be seen as experts with special knowledge of what to do in such situations. We argue that this model presents significant advantages to deal with complex problems such as vibration surveillance.     

Ultrafast imaging of polyatomic molecules with simplex algorithm

A simplex algorithm used to solve molecular structure from asymptotic momentum values is reported. The geometry of ${\text{CO}}_2^{3+}$ molecular ions in linear and bent configurations is reconstructed. A polyatomic molecular ion with trigonal planar symmetry is used to demonstrate the scalability of the algorithm.     

Biocompatibility of heparin-grafted hemodialysis membranes: impact on monocyte chemoattractant protein-1 circulating level and oxidative status

This prospective observational study aimed at evaluating efficacy and biocompatibility performances of the new heparin-coated Evodial dialyzers with/without systemic heparin reduction. After a 4-week wash-out period with reference polysulfone F70S dialyzers, 6 hemodialysis patients were sequentially dialyzed with Evodial, F70S, and Evodial dialyzers using 30% heparin reduction, each period of treatment was 4 weeks. Removal rates (RR) (urea, creatinine, and β2-microglobulin), dialysis dose, and instantaneous clearances (urea and creatinine) were measured as well as inflammatory (C-reactive protein, fibrinogen, interleukin 6, tumor necrosis factor α, and monocyte chemoattractant protein-1) and oxidative stress (OS) (superoxide anion, homocysteine, and isoprostanes) parameters at the end of each study period. Patients treated with Evodial or F70S dialyzers for 4 weeks presented comparable dialysis efficacy parameters including urea and creatinine RR, dialysis dose and instantaneous clearances. By contrast, a significantly lower but reasonably good β2-microglobulin RR was achieved with Evodial dialyzers. Regarding biocompatibility, no significant difference was observed with inflammation and OS except for postdialysis monocyte chemoattractant protein-1 which significantly decreased with Evodial dialyzers. Thirty percent heparinization reduction with Evodial dialyzers did not induce any change in inflammation but led to an improvement in OS as demonstrated by a decrease in postdialysis superoxide production and predialysis homocysteine and isoprostane. This bioactive dialyzer together with heparin dose reduction represents a good trade-off between efficacy and biocompatibility performance (improvement in OS with a weak decrease in efficacy) and its use is encouraging for hemodialysis patients not only in reducing OS but also in improving patient comorbid conditions due to lesser heparin side effects.     

Magnetically responsive polypyrrole nanotubes using Ce(III)-stabilized maghemite nanoparticles

Nanocomposites (NCs) that are made magnetically responsive in controlled conditions attract continuing interest for their added magnetic properties. In this study, we report on the preparation and full characterization of a multifunctional NC composed of magnetic γ-Fe(2)O(3) nanoparticles (NPs) covalently attached to the surface of polyaminated (polyNH(2)) poly(2,6-di-pyrrol-1-yl-hexanoic acid) (pDPL) nanotubes (NTs). Such a hybrid conducting polymer iron oxide maghemite γ-Fe(2)O(3)@pDPL NC built specifically on covalent bonding has never been reported. The maghemite γ-Fe(2)O(3) NPs were prepared using an innovative ultrasound-assisted Ce(3+) doping process, resulting in polycarboxylation of the NP surface useful for control of aggregation and derivatization of functionality. The second component of the NC, i.e. polyNH(2)-modified pDPL NTs, was prepared from an acid functional pyrrole species followed by amine modification. The resulting innovative γ-Fe(2)O(3)@pDPL NC can be viewed as a multifunctional nanomaterial since it possesses both types of derivatization, i.e. polyCOOH (NPs) and polyNH(2) (NTs) combined with magnetic responsivity.     

Does hemodiafiltration improve the removal of homocysteine?

High prevalence of hyperhomocysteinemia is common in hemodialysis (HD) patients and could contribute to worsen the cardiovascular risk. Beyond vitamin B status, dialysis modality itself could influence homocysteine (Hcy) levels. The objective was compare the reduction rate (RR) of Hcy and cysteine in stable dialyzed patients treated by standard HD or hemodiafiltration (HDF). Seventy‐five patients undergoing stable dialysis through standard high‐flux HD (n = 35) or HDF (n = 40) were included. Biological parameters were determined before and after a midweek dialysis session. Urea percent reduction per session and Kt/V index (K, body urea clearance, T, time of dialysis, and V, urea distribution volume), defined as a marker of dialysis efficacy, were similar between HD and HDF groups. By contrast, higher RR of beta2 microglobulin (β2m) was observed in HDF compared with HD (78.6 vs. 72.0%, respectively; P < 0.001). Likewise, higher RR of Hcy was obtained with HDF compared to HD (46.0 vs. 41.5%, respectively; P < 0.05), whereas the RR of cysteine was similar in both groups. Interestingly, a positive correlation between Hcy RR and urea Kt/V index was observed (r = 0.29, P < 0.05) and between Hcy RR and β2m RR (r = 0.45, P < 0.001). Time‐averaged concentration (TAC) of Hcy was lower with HDF compared with HD (17.8 vs. 19.1 μmol/L, respectively), although not significant. There was no difference in median Hcy according to dialysis modality for neither pre‐ nor postdialysis levels. Significant higher removal of Hcy was observed with HDF compared with standard HD, although urea Kt/V index was similar. Enhanced removal of middle molecules, such as β2m, could be involved in Hcy RR improvement with HDF.     

Electroless ultrasonic functionalization of diamond nanoparticles using aryl diazonium salts

We report a novel and very handful strategy for the functionalization of diamond nanoparticles (NDs), based on the ultrasound-assisted grafting of aryl groups from the electroless reduction of diazonium salts. For this study, 4-nitrobenzenediazonium salt was used as a model molecule and the reaction was investigated in neutral and acidic aqueous media. Spectroscopic evidence for the successful attachment of aryl groups to nanodiamonds (NDs) was given by IR and XPS which clearly detect characteristic NO₂ peaks. Moreover, the absence of any peaks from the ⁺N≡N group in the IR spectra is a clear indication of the chemical reduction of the parent diazonium salt at the surface of NDs. This spontaneous chemical modification of NDs by aryl diazonium salts was confined to the surface of the ND particles; indeed, XRD measurements have shown that the crystalline structure of the bulk of the particles was unaffected. It opens up new possibilities towards the control of the surface chemical composition of NDs using simple protocols operated in very soft conditions, i.e. in water at room temperature. It shows conclusively that the chemistry toolbox of experts interested in nanodiamonds should contain aryl diazonium salts, given their versatility in forming active platforms.     

Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells

Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.     

Libre parcours moyen dans la phase solide des materiaux frittés

A simple mathematical model is used to predict the evolution of the mean free path in the solid phase in a sintered material. The knowledge of the volume fraction of the solid phase and of the particle coordination number are only required. Good agreement is observed between experimental data and the purposed relationship.